Cloning of a cation efflux pump gene associated with chlorhexidine resistance in Klebsiella pneumoniae
Journal
Antimicrobial Agents and Chemotherapy
Journal Volume
46
Journal Issue
6
Pages
2024-2028
Date Issued
2002
Author(s)
Abstract
Expression libraries of a chlorhexidine-resistant Klebsiella pneumoniae strain were constructed and transformed into Escherichia coli XLOLR. Twenty chlorhexidine-resistant transformants were obtained after selection. All clones contained a novel 903-nucleotide locus. Its sequences were compatible with a cation efflux pump, and the locus was thus designated as cepA. Retransformation using cepA-containing plasmids conferred chlorhexidine resistance to both XLOLR and a chlorhexidine-sensitive K. pneumoniae strain. Therefore, CepA is associated with chlorhexidine resistance and may act as a cation efflux pump.
SDGs
Type
journal article