Electrochemical biosensor with electrokinetics-assisted molecular trapping for enhancing C-reactive protein detection
Journal
Biosensors and Bioelectronics
Journal Volume
210
Date Issued
2022
Author(s)
Abstract
C-Reactive protein (CRP) is an essential biomarker relevant to various disease prognoses. Current biosensors require a significant amount of time for detecting CRP. To address this issue, this work proposes electrokinetic flow-assisted molecule trapping integrated with an impedance biosensor, where a driving signal in terms of a gated sine wave is provided to circularly arranged electrodes which detect proteins. To verify the biosensor's efficacy, protein aggregation on the electrode surface was evaluated through a fluorescence analysis and measurement of the electrochemical impedance spectrum (EIS). The fluorescence analysis with avidin showed that target samples largely accumulated on the electrode surface upon provision of the driving signal. The EIS measurement of CRP accumulation on the electrode surface further confirmed a significant electrokinetic phenomenon at the electrode/electrolyte interface. Even at the low CRP concentration of 10 pg/ml, the proposed device's sensitivity and reliability were as high as 3.92 pg/ml with a signal-to noise ratio (SNR) of ≥3, respectively. In addition, the protein detection time (without considering the preparation time) was minimized to as low as 90 s with the proposed device. This device's advantage is its minimal time consumption, and simple drop-analysis process flow; hence, it was used for monitoring clinical serum samples. © 2022 Elsevier B.V.
Subjects
C-Reactive protein; Dielectrophoresis; Electro-thermal convection; Electrochemical impedance spectroscopy (EIS); Preconcentration
SDGs
Other Subjects
Biosensors; Chemical detection; Diagnosis; Electrochemical electrodes; Electrodynamics; Electrohydrodynamics; Electromagnetic fields; Electrophoresis; Fluorescence; Heat convection; Microfluidics; Proteins; Signal to noise ratio; C-reactive proteins; Driving signal; Electro-thermal convection; Electrochemical impedance spectroscopy; Electrochemical-impedance spectroscopies; Electrode surfaces; Fluorescence analysis; Pre-concentration; Protein detection; Thermal convections; Electrochemical impedance spectroscopy; C reactive protein; electrolyte; C reactive protein; Article; concentration (parameter); dielectrophoresis; fluorescence analysis; impedance spectroscopy; limit of detection; protein aggregation; protein analysis; reliability; sensitivity analysis; signal noise ratio; surface property; thermodynamics; electrochemical analysis; electrode; genetic procedures; reproducibility; Biosensing Techniques; C-Reactive Protein; Electrochemical Techniques; Electrodes; Reproducibility of Results
Type
journal article