The role of peroxisome proliferater activated receptor γ (PPARγ) in thioacetamide-induced hepato-steatosis of zebrafish（Danio rerio）
Date Issued
2006
Date
2006
Author(s)
Wang, Chi An
DOI
zh-TW
Abstract
Abstract：
Peroxisome proliferator-activated receptor gamma (PPARγ) is considered to be one of the master regulators of adipocyte differentiation. In recent report, liver-specific disruption of PPARgamma improves fatty liver in leptin-dificient mice（Matsusue et al.,2003）. PPARγ2 is abundantly expressed in mature adipocytes and is elevated in the liver of animals that develop fatty liver. PPARγ regulates lipogenesis and lipid accumulation in steatotic hepatocyte. The aim of this study was to study the role of PPARγin pathogenesis of fatty liver. And to examine over-expression of PPARγ in liver by liver-specific L-FABP promoter and ubiquitous expression by EF-1αpromoter could induce lipogenesis and result in steatosis ? To understand roles of PPARγ in normal and fatty liver in zebrafish, I first cloned two cDNAs with length of 1533 bp and 1581 bp encoding PPARγ1 and PPARγ2, respectively. Liver-specific over-expression of PPARγ in zebrafish larva resulted in up-regulation of adipogenetic genes such as, adipocyte Fatty Acid Binding Protein (AFABP/AP2), adiponectin, and lipogenetic gene such as, SREBP-1. Secondly, I tried to promote hepatosteatosis in PPARγ-injected 5 day post fertilization (dpf) zebrafish larva by hepatotoxin thioacetamid (TAA) treatment. We have previously showed that TAA treatment for 7 days leads to liver steatohepatitis of zebrafish larva at 10 dpf. After injected with pLF2.8-zfPPARγ, zebrafish larva at 5 dpf were treated with TAA for 10 days. The results of RT-PCR showed that zebrafish treated with TAA had adipogenetic and lipogenetic genes up-regulated on the third and 5th day, After injected pLF2.8-zfPPARγ 5-day postfertilization, treaded TAA 10 days. The results of RT-PCR show that zebrafish treaded TAA 10th day made adipogenetic and lipogenetic up-regulated on the 3th and 5th day, because on TAA treated control Data show that lipogenetic gene would up-regulate at 9th day, so overexpression of PPARγ enhance TAA induced fatty liver.
Subjects
斑馬魚
肝臟
zebrafish
liver
SDGs
Type
other
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