Differentiation of bacteria cell wall using Raman scattering enhanced by nanoparticle array
Journal
Journal of Nanoscience and Nanotechnology
Journal Volume
12
Journal Issue
6
Pages
5004-5008
Date Issued
2012
Author(s)
Liu, T.-Y.
Chen, Y.
Wang, H.-H.
Huang, Y.-L.
Chao, Y.-C.
Tsai, K.-T.
Cheng, W.-C.
Chuang, C.-Y.
Tsai, Y.-H.
Huang, C.-Y.
Wang, D.-W.
Lin, C.-H.
Wang, Y.-L.
Abstract
We have fabricated surface-enhanced Raman scattering (SERS) substrates based on arrays of silver nanoparticles grown on porous anodic alumina templates. Using this nanotechnology platform, label-free and high-speed detection of bacteria are achieved. SERS spectra of various bacteria including Staphylococcus Aureus (Gram-positive bacterium), Klebsiella Pneumoniae (Gram-negative bacterium), and Mycobacterium Smegmatis (Mycobacterium) were recorded. The highly reproducible SERS-based technological platform is capable of differentiating different kinds of bacteria by PCA, LDA, clustering analysis, and SVM methods, which provides promising opportunity for biosensing of clinical microbes. Copyright © 2012 American Scientific Publishers. All rights reserved.
Publisher
Ingenta
Type
journal article