Determination of Ochratoxin A in Coffee Drinks using Immunoaffinity Cleanup and Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry with Isotope-Dilution Techniques
Date Issued
2011
Date
2011
Author(s)
Chang, Chiung-Wen
Abstract
Ochratoxin A (OTA) is common contaminant in food resulting from a secondary metabolite of fungi, and the International Agency for Research on Cancer (IARC) has classified OTA as a possible human carcinogen (2B). The processes of baking coffee beans are not able to remove OTA completely. Humans are exposed to OTA via drinking coffee drinks.
This study developed and validated a method to determine the concentrations of OTA in coffee drinks using immunoaffinity column extraction and detection of ultra-high performance liquid chromatography/tandem mass spectrometry (UHPLC-MS/MS) with isotope-dilution techniques by 13C20-OTA as the internal standard. The OTA was analyzed on an Ascentis Express RP-amide column (50
Subjects
Ochratoxin A
immunoaffinity column
UHPLC-MS/MS
coffee
SDGs
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-100-R98844006-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):6f7431e082eefdd148bb232f2cbaa806