In vivo sub-femtoliter resolution photoacoustic microscopy with higher frame rates
Journal
Scientific Reports
Journal Volume
5
ISSN
20452322
Date Issued
2015-10-21
Author(s)
Abstract
Microscopy based on non-fluorescent absorption dye staining is widely used in various fields of biomedicine for 400 years. Unlike its fluorescent counterpart, non-fluorescent absorption microscopy lacks proper methodologies to realize its in vivo applications with a sub-femtoliter 3D resolution. Regardless of the most advanced high-resolution photoacoustic microscopy, sub-femtoliter spatial resolution is still unattainable, and the imaging speed is relatively slow. In this paper, based on the two-photon photoacoustic mechanism, we demonstrated a in vivo label free laser-scanning photoacoustic imaging modality featuring high frame rates and sub-femtoliter 3D resolution simultaneously, which stands as a perfect solution to 3D high resolution non-fluorescent absorption microscopy. Furthermore, we first demonstrated in vivo label-free two-photon acoustic microscopy on the observation of non-fluorescent melanin distribution within mouse skin.
Other Subjects
melanin; animal; isolation and purification; metabolism; microscopy; mouse; photoacoustics; procedures; skin; ultrastructure; Animals; Melanins; Mice; Microscopy; Photoacoustic Techniques; Skin
Publisher
Nature Publishing Group
Type
journal article