Application of Two-Step Drug Release System in Adipose Tissue Engineering
Date Issued
2009
Date
2009
Author(s)
Wu, I-Chen
Abstract
Recent studies in adipose tissue engineering have indicated that in vivo adipogensis requires an appopriate cell source, scaffold and growth factors. Preadipocytes, precursor cells committed to the adipocyte lineage are the ideal cell source. They are capable of proliferating to obtain extensive cell numbers in vitro and differenting into mature fat cells. Once cells differentiate, they are not capable of replication. Therefore, in order to induce the formation of larger fat tissue, it is essential to increase the numbers of preadipocytes before differentiation. readipocytes proliferate and differentiate in the presence of the growth factors. In this study, we found that 0.5 ng/mL bFGF is able to accelerate cell proliferation in vitro, and insulin and dexamethasone enable preadipocytes to differentiate according to the previous study. In order to release two types of growth factor in two-stage, we develop a method to obtain the core-shell particles with insulin/dex in the PDLLA core and gelatin shell layer for bFGF sorption. Core-shell particles are prepared for the controlled release of bFGF and followed by insulin/dex. The proliferation ability of 3T3-L1 incorporated by core-shell particles containing bFGF in vitro is assessed by the MTS assay. The MTS result shows that the controlled release of bFGF increases the number of preadipocytes. By injecting two types of scaffold, collagen/HA and gelatin microspheres respectively, in combination with core-shell particles and preadipocytes into the back of the Balb/c mice, the adipogenesis at the subcutasneous implantation sites is evalutated histologically.In conclusion, PDLLA particles (core) coating with gelatin (shell) are successfully acheived and ued as the two-step controlled release system in the application of adipose tissue engineering. The first step controlled release of bFGF increases the proliferation rate of 3T3-L1. The combination of preadipocytes and two-step drug release system with scaffold has the potential for augmentation of adipose tissue.
Subjects
adipose tissue engineering
gelatin
polylactic acid
microsphere
core-shell particle
basic fibroblast growth factor
Type
thesis
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