HRad17 colocalizes with NHP2L1 in the nucleolus and redistributes after UV irradiation
Journal
Journal of Biological Chemistry
Journal Volume
274
Journal Issue
51
Pages
36544 - 36549
Date Issued
1999
Author(s)
Sasaki, Hidefumi
Campbell, Michael S.
Kraeft, Stine-Kathrein
Sutherland, Rebecca
Yang, Chin-Yu
Liu, Yuan
Auclair, Daniel
Hao, Luning
Sonoda, Hikaru
Ferland, Louis H.
Chen, Lan Bo
Abstract
The rad17 gene of Schizosaccharomyces pombe plays an important role as a checkpoint protein following DNA damage and during DNA replication. The human homologue of S. pombe rad17, Hrad17, was recently identified, but its function has not yet been established. Using the yeast two-hybrid system, we determined that HRad17 can interact with a nucleolar protein, NHP2L1. This interaction was also demonstrated biochemically, in human cells. Immunofluorescence studies revealed that HRad17 and NHP2L1 colocalize to the nucleolus, and immunogold labeling further resolved the location of NHP2L1 to the dense fibrillar component of the nucleolus. Interestingly, the localization of HRad17 in the nucleolus was altered in response to UV irradiation. These results provide some insight into the DNA damage and replication checkpoint mechanisms of HRad17.
Type
journal article