In vivo track the development of melanoma with the intrinsic third harmonic generation and two-photon fluorescence contrasts of melanin
Journal
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Journal Volume
8233
Date Issued
2012
Author(s)
Abstract
The understanding of the interaction between tumors and surrounding microenvironment in vivo is an important first step and basis for pathway-targeting cancer therapy. To in vivo observe the dynamic development of tumor cells and validate the efficacy of therapy in microscopic scales, people commonly performed multi-photon fluorescence microscopy through an invasive window chamber setup. However, under such system, the cancer cells can't be identified and long-term tracked without a fluorescence labeling. Exploiting the intrinsic third harmonic generation (THG) and two-photon fluorescence (2PF) contrasts of melanin, we demonstrated in vivo identification of melanoma and tracked its development without labeling. It was achieved with a least invasive femtosecond Cr:forsterite laser and a laser scanning nonlinear microscopy system with 3D sub-micron spatial resolution. Combined with molecular probes or reporters, we anticipate thus developed platform a powerful tool to reveal molecular insights of tumor microenvironments, enhance our understanding of tumor biology, and trigger new therapeutic approaches. ? 2012 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE).
Subjects
Cell tracking; harmonic generation microscopy; melanoma; tumor microenvironments; two-photon fluorescence
SDGs
Other Subjects
Cell tracking; harmonic generation microscopy; melanoma; Microenvironments; two-photon fluorescence; Dermatology; Fluorescence; Fluorescence microscopy; Harmonic generation; Medical applications; Nanoparticles; Photons; Probes; Tumors; Oncology
Type
conference paper