Species distribution and antifungal susceptibilities of clinical isolates of Penicillium and Talaromyces species in China
Journal
International journal of antimicrobial agents
Journal Volume
58
Journal Issue
1
Date Issued
2021-07
Author(s)
Guo, Li-Na
Yu, Shu-Ying
Wang, Yao
Liu, Ya-Li
Yuan, Ying
Duan, Si-Meng
Yang, Wen-Hang
Jia, Xin-Miao
Zhao, Ying
Xiao, Meng
Xie, Xiu-Li
Dou, Hong-Tao
Xu, Ying-Chun
Abstract
Morphologically identified Penicillium (n = 103) and Talaromyces marneffei (n = 8) isolates were collected from various clinical sources between 2016 and 2017 at a medical centre in Beijing, China. Identification to species level was confirmed by sequencing of the internal transcribed spacer (ITS) region, β-tubulin gene (benA) and RNA polymerase II second largest subunit (RPB2) gene. Of the 111 isolates, 56 (50.5%) were identified as Penicillium spp. and 55 (49.5%) as Talaromyces spp. Eleven species of Penicillium were detected, of which Penicillium oxalicum was the commonest, accounting for 51.8% (29/56), followed by Penicillium rubens (10.7%; 6/56) and Penicillium citrinum (10.7%; 6/56). Among the 55 Talaromyces isolates, nine species were identified, with Talaromyces funiculosus (36.4%; 20/55), Talaromyces stollii (27.3%; 15/55) and Talaromyces marneffei (14.5%; 8/55) being the most common. Of note, 89.3% (50/56) of the Penicillium isolates and 98.2% (54/55) of the Talaromyces isolates exhibited growth at 37°C. The isolates were mainly recovered from patients with pulmonary disorders (56.8%; 63/111), autoimmune disease (12.6%; 14/111) and AIDS (5.4%; 6/111). The azoles and amphotericin B exhibited potent activity against T. marneffei, while various levels of activity were observed against Penicillium and other Talaromyces species The echinocandins had the lowest MECs (MEC90, ≤0.12 mg/L) against most Penicillium and Talaromyces species, with the exception of T. marneffei whose MEC90 (4 mg/L) was five or more dilutions higher than that of the other species tested. These data on the species distribution and antifungal susceptibility expand the current clinical knowledge of Penicillium and Talaromyces species.
Subjects
Antifungal susceptibility; Internal transcribed spacer region; Penicillium; RNA polymerase II second largest subunit gene; Talaromyces; β-Tubulin
Other Subjects
amphotericin B; echinocandin; pyrrole derivative; antifungal agent; fungal DNA; mycosis; RNA polymerase II; tubulin; antifungal susceptibility; Article; benA gene; China; Eurotiales; fungal gene; fungal strain; fungus growth; fungus isolation; MIC90; nonh
Type
journal article