Hydrostatic pressure promotes migration and filamin-A activation in fibroblasts with increased p38 phosphorylation and TGF-β production
Journal
Biochemical and Biophysical Research Communications
Journal Volume
568
Pages
15-22
Date Issued
2021
Author(s)
Abstract
Fibroblast migration is closely regulated by the mechanical characteristics in surrounding microenvironment. While increased interstitial hydrostatic pressure (HP) is a hallmark in many pathological and physiological conditions, little is known about how the HP affects fibroblast motility. Using cell-culture chips with elevated HP conditions, we showed that 20 cmH2O HP significantly accelerated fibroblast migration. The HP-induced migration acceleration was dependent on the augmentation of transforming growth factor-β1, and correlated with the activation of filamin A via the phosphorylation of p38 mitogen-activated protein kinase. Our results suggest that interstitial HP elevation associated with various pathological states could significantly regulate fibroblast migration. ? 2021 The Authors
Subjects
Cell motility
Fibroblast
Filamin
Interstitial hydrostatic pressure
p38
TGF-β
filamin A
mitogen activated protein kinase p38
transforming growth factor beta
filamin
FlnA protein, mouse
Article
cell culture
cell migration
cell motility
controlled study
fibroblast
hydrostatic pressure
MAPK signaling
NIH 3T3 cell line
protein expression
protein function
protein phosphorylation
protein secretion
upregulation
animal
cell motion
cytology
metabolism
mouse
phosphorylation
Animals
Cell Movement
Fibroblasts
Filamins
Hydrostatic Pressure
Mice
NIH 3T3 Cells
p38 Mitogen-Activated Protein Kinases
Phosphorylation
Transforming Growth Factor beta
SDGs
Type
journal article