Hydrodynamic HBV transfection mouse model
Journal
Methods in Molecular Biology
Journal Volume
1540
Pages
227-235
Date Issued
2017
Author(s)
Abstract
Hydrodynamic HBV transfection mouse model is an established method of the last decade where macromolecules, non-normally permeable to cell membrane, are delivered intracellular. The basic principle is that a large volume of solution, containing HBV plasmid construct, is infused rapidly in circulation to permit the preferential entrance of these macromolecules to liver parenchymal cells. The aim of this chapter is to describe the basic principles of the hydrodynamic HBV transfection in mouse models.
SDGs
Publisher
Humana Press Inc.
Type
book part