Disease-specific B cell epitopes for serum antibodies from patients with Severe Acute Respiratory Syndrome (SARS) and serologic detection of SARS antibodies by epitope-based peptide antigens
Journal
Journal of Infectious Diseases
Journal Volume
190
Journal Issue
4
Pages
797-809
Date Issued
2004
Author(s)
Abstract
Severe acute respiratory syndrome (SARS) has emerged as a highly contagious, sometimes fatal disease. To find disease-specific B cell epitopes, phage-displayed random peptide libraries were panned on serum immunoglobulin (Ig) G antibodies from patients with SARS. Forty-nine immunopositive phage clones that bound specifically to serum from patients with SARS were selected. These phageborne peptides had 4 consensus motifs, of which 2 corresponded to amino acid sequences reported for SARS-associated coronavirus (SARS-CoV). Synthetic peptide binding and competitive-inhibition assays further confirmed that patients with SARS generated antibodies against SARS-CoV. Immunopositive phage clones and epitope-based peptide antigens demonstrated clinical diagnostic potential by reacting with serum from patients with SARS. Antibody-response kinetics were evaluated in 4 patients with SARS, and production of IgM, IgG, and IgA were documented as part of the immune response. In conclusion, B cell epitopes of SARS corresponded to novel coronavirus. Our epitope-based serologic test may be useful in laboratory detection of the virus and in further study of the pathogenesis of SARS.
SDGs
Other Subjects
epitope; immunoglobulin A; immunoglobulin G; immunoglobulin G antibody; immunoglobulin M; peptide library; virus antibody; amino acid sequence; antibody blood level; antibody detection; antibody production; antibody response; antigen binding; article; B lymphocyte; binding assay; blood examination; clinical article; clone; competitive inhibition; concentration response; controlled study; human; human cell; immune response; immunogenicity; immunoglobulin production; nucleotide sequence; peptide analysis; phage display; priority journal; SARS coronavirus; severe acute respiratory syndrome; Amino Acid Motifs; Amino Acid Sequence; Animals; Antibodies, Viral; Antigen-Antibody Reactions; Antigens, Viral; B-Lymphocytes; Cercopithecus aethiops; Epitopes; Humans; Molecular Sequence Data; Peptide Library; Peptides; SARS Virus; Sequence Alignment; Severe Acute Respiratory Syndrome; Species Specificity; Vero Cells
Type
journal article