Structural and biological insights into Klebsiella pneumoniae surface polysaccharide degradation by a bacteriophage K1 lyase: implications for clinical use
Journal
Journal of biomedical science
Journal Volume
29
Journal Issue
1
Date Issued
2022-02-07
Author(s)
Tu, I-Fan
Lin, Tzu-Lung
Yang, Feng-Ling
Lee, I-Ming
Tu, Wei-Lin
Liao, Jiahn-Haur
Ko, Tzu-Ping
Wu, Wen-Jin
Jan, Jia-Tsrong
Ho, Meng-Ru
Chou, Ching-Yi
Wang, Andrew H-J
Wu, Chung-Yi
Huang, Kai-Fa
Wu, Shih-Hsiung
Abstract
K1 capsular polysaccharide (CPS)-associated Klebsiella pneumoniae is the primary cause of pyogenic liver abscesses (PLA) in Asia. Patients with PLA often have serious complications, ultimately leading to a mortality of ~ 5%. This K1 CPS has been reported as a promising target for development of glycoconjugate vaccines against K. pneumoniae infection. The pyruvylation and O-acetylation modifications on the K1 CPS are essential to the immune response induced by the CPS. To date, however, obtaining the fragments of K1 CPS that contain the pyruvylation and O-acetylation for generating glycoconjugate vaccines still remains a challenge.
Subjects
Acetylation; K1 capsular polysaccharide; Klebsiella pnuemoniae; Polysaccharide lyase; Pyruvylation; Tailspike protein; β-Helix
SDGs
Type
journal article