The Metabolic Benefits of Polygonum hypoleucum Ohwi in HepG2 Cells and Wistar Rats under Lipogenic Stress
Resource
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 58(8), 5174-5180
Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Journal Volume
58
Journal Issue
8
Pages
5174-5180
Date Issued
2010
Date
2010
Author(s)
Chao, Pei-Min
Kuo, Yueh-Hsiung
Lin, Yu-Shun
Chen, Chi-Hua
Chen, Shiow-Wen
Kuo, Yao-Haur
Abstract
Inhibition of acetyl-CoA carboxylase (ACC) is one approach used for treating metabolic syndrome. Using partially purified ACC to screen herbs commonly used in Taiwanese folk medicine, we previously showed that an ethanol extract of Polygonum hypoleucum Ohwi (EP) had potent ACC inhibitory activity and partially alleviated metabolic disorders induced by a high fat diet. Since ACC plays a crucial role in de novo lipogenesis, the favorable effects of EP on metabolism were tested under lipogenic conditions in the present study. On incubating high glucose (30 mM)-stimulated HepG2 cells with EP (72.5 or 145 μg/mL), ACC and fatty acid synthase activity, triacylglycerol content, and microsomal triacylglycerol transfer protein mRNA levels were all significantly reduced (P < 0.05, vs vehicle). When EP was given at low, medium, and high dosages (94, 188, and 470 mg/kg) to sucrose water-treated Wistar rats for four weeks, alleviation of symptoms associated with metabolic syndrome, including obesity, insulin resistance, hypertriglyceridemia, and hypertension, accompanied by hepatic ACC inactivation, was seen in the low dosage group. Four compounds (emodin, emodin-8-O-β-d-glucopyranoside, (+)-catechin, and (-)-epicatechin) isolated from EP were identified as ACC inhibitors. These results confirm that P. hypoleucum Ohwi, acting partly through ACC inhibition, has favorable effects in alleviating metabolic disturbances occurring under lipogenic conditions. ? 2010 American Chemical Society.
Subjects
Acetyl-CoA carboxylase; Lipogenic condition; Metabolic syndrome; P. hypoleucum Ohwi
SDGs
Other Subjects
acetyl coenzyme A carboxylase; carrier protein; fatty acid synthase; messenger RNA; microsomal triglyceride transfer protein; plant extract; triacylglycerol; animal; article; cell line; chemistry; genetics; human; lipid metabolism; metabolism; Polygonum; rat; Wistar rat; Acetyl-CoA Carboxylase; Animals; Carrier Proteins; Cell Line; Fatty Acid Synthetase Complex; Humans; Lipid Metabolism; Plant Extracts; Polygonum; Rats; Rats, Wistar; RNA, Messenger; Triglycerides; Polygonum; Polygonum hypoleucum; Rattus norvegicus
Type
journal article
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