Electrophysiological Mechanisms for the Antiarrhythmic Activities of Naloxone on Cardiac Tissues
Resource
LIFE SCIENCES v.63 n.14 pp.1205-1219
Journal
LIFE SCIENCES
Journal Volume
v.63
Journal Issue
n.14
Pages
1205-1219
Date Issued
1998
Date
1998
Author(s)
HUNG, CHI-FENG
WU, MEI-HWAN
TSAI, CHANG-HER
CHU, SHU-HSUN
CHI, JO-FENG
SU, MING-JAI
Abstract
It has been reported that naloxone, an opioid antagonist, has antiarrhythmic activity in vivo. In Langendorff perfused rat hearts, we found that ischemia-reperfusion-induced ventricular tachyarrhythmia reverted to normal sinus rhythm after the treatment with naloxone (3 similar to 10 mu M). The method of voltage and current clamp were used to study the underlying mechanism of its antiarrhythmic activity on isolated cardiac myocytes. In isolated rat ventricular and in guinea-pig and human atrial myocytes, naloxone prolonged the action potential duration reversibly. In rat ventricular myocytes, naloxone (1 similar to 30 mu M) inhibited sodium current (I-Na), transient outward potassium current (I-to ), and calcium current (I-Ca). On the contrary, the addition of naloxone significantly increased inward rectifier potassium current (I-Kl) For the effect on I-Na, naloxone did not shift the inactivation curve of I-Na but retarded the I-Na recovery rate from inactivation state. Naloxone suppressed I -to with a significant left-shift of the inactivation curve, however, the time course of I-to recovery from inactivation was not affected. In guinea pig atrial myocytes, naloxone ( 10 mu M) decreased the delayed rectifier K+ current (I-K) These results show that naloxone exert various extent of inhibition on I-Na, I-to, I-K and I-Ca. The prolongation of cardiac action potential is related to the inhibition of I - to and I-K. The antiarrhythmic activity of naloxone is more closely related to the inhibition of Na+ and K+ currents rather than the blockade of myocardial opioid receptors.
Type
journal article