Glycan Microarray of Globo H and Related Structures for Quantitative Analysis of Breast Cancer
Resource
Proceedings of the National Academy of Science, 105(33), 11661-11666
Journal
Proceedings of the National Academy of Sciences
Pages
11661-11666
Date Issued
2008
Date
2008
Author(s)
Wang, Cheng-Chi
Ren, Chien-Tai
Lin, Chin-Wei
Hung, Jung-Tung
Yu, Jyh-Cherng
Yu, Alice L.
Wu, Chung-Yi
Wong, Chi-Huey
Abstract
Cancer-associated carbohydrate antigens are often found on the surface of cancer cells. Understanding their roles in cancer progression will lead to the development of new therapeutics and high-sensitivity diagnostics for cancers. Globo H is a member of this family, which is highly expressed on breast cancer cells. Here, we report the development of a glycan microarray of Globo H and its analogs for measurement of the dissociation constants on surface (K D,surf) with three different monoclonal antibodies (VK-9, Mbr1, and anti-SSEA-3), to deduce their binding specificity. The glycan microarray was also used to detect the amount of antibodies present in the plasma of breast cancer patients and normal blood donors. It was shown that the amount of antibodies against Globo H from breast cancer patients were significantly higher than normal blood donors, providing a new tool for possible breast cancer diagnosis. Compared with the traditional ELISA method, this array method required only atto-mole amounts of materials and is more effective and more sensitive (5 orders of magnitude). The glycan microarray thus provides a new platform for use to monitor the immune response to carbohydrate epitopes after vaccine therapy or during the course of cancer progression. ? 2008 by The National Academy of Sciences of the USA.
Subjects
Array; Diagnosis
SDGs
Other Subjects
carbohydrate antigen; fucose; globo h antigen; glycan; monoclonal antibody; monoclonal antibody mbr 1; monoclonal antibody ssea 3; monoclonal antibody vk 9; polyclonal antibody; animal experiment; animal model; article; breast cancer; cancer diagnosis; controlled study; enzyme linked immunosorbent assay; female; human; immune response; major clinical study; microarray analysis; mouse; nonhuman; priority journal; protein binding; protein interaction; quantitative analysis; Animals; Antibodies; Antibody Specificity; Antigens, Tumor-Associated, Carbohydrate; Breast Neoplasms; Carbohydrate Conformation; Carbohydrate Sequence; Enzyme-Linked Immunosorbent Assay; Health; Humans; Mice; Microarray Analysis; Polysaccharides; Surface Properties
Type
journal article
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