A Simple and Rapid Test-card Method to Detect Hepatitis B Surface Antigen and Antibody: Potential Application in Young Children and Infants
Journal
Pediatrics and Neonatology
Journal Volume
57
Journal Issue
3
Pages
219-224
Date Issued
2016
Author(s)
Abstract
Background Hepatitis B surface antigen (HBsAg) and hepatitis B surface antibody (anti-HBs) were conventionally determined by enzyme immunoassays. We aimed to apply a rapid, simple, and accurate method to detect HBsAg and its antibody. Methods We collected 1463 serum samples from healthy volunteers, hepatitis B carriers, and children of HBsAg-positive mothers. The test card that we examined is a chromatographic immunoassay for the qualitative detection of either HBsAg or anti-HBs. We then compared the results of the test card to the results of the conventional enzyme-immunoassay method, which is regarded as a standard. Results In the use of the test card to check HBsAg, the sensitivity was 88.8% and the specificity was 100%. The median hepatitis B virus viral load was significantly higher in the true-positive group [103.71 copies/mL (range, 102-109.03 copies/mL)] than in the false-negative group [102 copies/mL (range, 102-103.26 copies/mL)] (p = 0.005). In those who were younger than 2 years, the diagnostic accuracy of the HBsAg test card was 100%. Then, 1272 samples were tested for anti-HBs rapid test card. The sensitivity was 91.8% and the specificity was 96.5%. The median anti-HBs titer was significantly higher in the true-positive group (295.8 mIU/mL) than in the false-negative group (42.3 mIU/mL; p < 0.001). Conclusion Because of (1) the limited amount of blood sample required and (2) most of the young hepatitis B virus carriers having high viremia, and no concerns of false negativity, the test card is a good rapid screening tool for the detection of HBsAg and anti-HBs in pediatric group. ? 2015, Taiwan Pediatric Association. Published by Elsevier Taiwan LLC. This is an open access article under the CC BY-NC-ND license.
SDGs
Other Subjects
hepatitis B surface antibody; hepatitis B surface antigen; virus DNA; hepatitis B antibody; hepatitis B surface antigen; antibody detection; antigen detection; Article; blood sampling; child; controlled study; diagnostic accuracy; diagnostic test accuracy study; enzyme immunoassay; female; hepatitis B; hepatitis B rapid test; Hepatitis B virus; human; immunoaffinity chromatography; infant; major clinical study; male; sensitivity and specificity; viremia; virus carrier; virus load; blood; comparative study; enzyme linked immunosorbent assay; immunoassay; Taiwan; Enzyme-Linked Immunosorbent Assay; Female; Hepatitis B Antibodies; Hepatitis B Surface Antigens; Humans; Immunoassay; Infant; Male; Sensitivity and Specificity; Taiwan
Publisher
Elsevier (Singapore) Pte Ltd
Type
journal article