Applications of Coumarin Oligomer, Silica and Gold Nanoparticle to Sensors for Saccharide Receptors
Date Issued
2004
Date
2004
Author(s)
Liao, Yuan-Cheng
DOI
zh-TW
Abstract
Abstract
This dissertation goal is development of sugar receptor(protein) sensors. The skeleton of sensors include monomer and oligomer of coumarin, gold nanoparticle and silica nanoparticle. These skeleton further combine with some sugar residue, then sensor can enable to bind with analytic protein via sugar and protein specific binding ability, finally achieve the detective goal through readout unit. Because of the sensor system have signal readout unit themselves, so can ease to detect for analytic protein that label fluorescent molecule unnecessary. We develop these sensor system for improving the proteomic research efficiency.
We design and synthesis of sensor monomer consist of three components, including norbornene, coumarin and sugar moiety. We can construct of multivalent sugar-coumarin oligomer high efficiency via norbornene ring opening metathesis polymerization (ROMP). Alkene crown derivative lead into copolymer for increasing water solubility of oligomer and decreasing steric crowded of sugar residue. As a result of multivalent sugar residue and triethylene glycol construction for oligomer then enhance to bind with protein ability and raise detective limitation. The sensors utilize sugar residue to bind with multi-binding site of lectin specifically, then make interoligomeric cross-aggregation and precipitation. Consequently, we can reach detective goal by UV-visible absorbance value go down substantially.
In a part of gold nanoparticle application in sensor of sugar receptor, we expect to construct microarray characteristic sensor. This research focus on fabricate self-assemble gold nanoparticle monolayer on cover glass slide, then we design and synthesis of sugar disulfide derivatives immobilize on gold nanoparticle monolayer that can get sugar receptor sensor. When biomolecule attach and bind with sugar moiety that further measure absorbance signal variation of gold nanoparticle monolayer on glass. So we can use these sensor to determine protein binding with sugar moiety or not in real time. We successful construct sugar receptor sensors of binding with mannose, galactose and glucosamine specifically. We also fabricate two different sugar ligand area on identical glass, similarly, have good detective ability and specific recognition for two different lectins.
In a part of silica nanoparticle application in sensor of sugar receptor, we modified sugar-coumarin derivatives on silica nanoparticle surface. Equally, when multi-binding site of lectin and bacteria bind with sugar residue specifically of modification on silica nanoparticle surface, then produce silica nanoparticle cross-linking with biomolecule and make precipitable phenomenon in assay solution. We can ease diagnose protein and bacteria existence via naked eye. The silica nanoparticle surface modified mannose residue that can diagnose Salmonella typhimurium and Serratia marcescens. This silica nanoparticle detective limitation is about 105/mL for above bacillus.
Subjects
凝集素
金奈米粒子
香豆素
二氧化矽奈米粒子
silica nanoparticle
coumarin
gold nanoparticle
lectin
Type
thesis
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