Label free sub-picomole level DNA detection with Ag nanoparticle decorated Au nanotip arrays as surface enhanced Raman spectroscopy platform
Journal
Biosensors and Bioelectronics
Journal Volume
26
Journal Issue
5
Pages
2413-2418
Date Issued
2011
Author(s)
Abstract
Label free optical sensing of adenine and thymine oligonucleotides has been achieved at the sub-picomole level using self assembled silver nanoparticles (AgNPs) decorated gold nanotip (AuNT) arrays. The platform consisting of the AuNTs not only aids in efficient bio-immobilization, but also packs AgNPs in a three dimensional high surface area workspace, assisting in surface enhanced Raman scattering (SERS). The use of sub-10. nm AgNPs with optimum inter-particle distance ensures amplification of the chemically specific Raman signals of the adsorbed adenine, thymine, cytosine and guanine molecules in SERS experiments. High temporal stability of the Raman signals ensured reliable and repeatable DNA detection even after three weeks of ambient desk-top conservation. This facile architecture, being three dimensional and non-lithographic, differs from conventional SERS platforms. ? 2010 Elsevier B.V.
Subjects
Biosensor; DNA; Nanostructure; Surface enhanced Raman spectroscopy; Surface plasmon
SDGs
Other Subjects
Ag nanoparticle; Bio-immobilization; DNA detection; Guanine molecules; High surface area; High temporal stability; Interparticle distances; Label free; Optical sensing; Raman signal; Self-assembled; Silver nanoparticles; Surface enhanced Raman spectroscopy; Surface plasmon; Surface-enhanced Raman scattering; Architectural acoustics; Biosensors; DNA; Gold; Light transmission; Nanoparticles; Nanotips; Oligonucleotides; Plasmons; Raman spectroscopy; Signal detection; Silver; Three dimensional; Raman scattering; adenine; cytosine; gold; guanine; silver nanoparticle; thymine; article; chemical structure; DNA determination; immobilization; nanoarray; optical biosensor; Raman spectrometry; reliability; reproducibility; surface property; Biosensing Techniques; DNA; Equipment Design; Equipment Failure Analysis; Microchemistry; Nanoparticles; Nanotechnology; Oligonucleotide Array Sequence Analysis; Silver; Spectrum Analysis, Raman; Staining and Labeling
Publisher
Biosens Bioelectron
Type
journal article