SAMPLING PERFORMANCE FOR BIOAEROSOLS BY FLOW CYTOMETRY WITH FLUOROCHROME
Date Issued
2005-07-31
Date
2005-07-31
Author(s)
李芝珊
DOI
932320B002050
Abstract
Although culture-based analysis
remains the primary method for
environmental bioaerosol analysis, for better
understanding and quantifying of bioaerosols,
both culture and nonculture-based methods
should be used and compared. Here, flow
cytometry with fluorochrome (FCM/FL) was
applied to evaluate the sampling
performance of impingement (AGI-30
all-glass impinger) and filtration
(track-etched polycarbonate filter) with
different types of fluorescent dye staining
(cell membrane integrity and metabolism)
and then compared with a traditional culture
method (culturability). Two bacterial
aerosols (Escherichia coli and endospores of
Bacillus subtilis) and two fungal aerosols
(Candida famata and Penicillium citrinum
spores) were studied. The bioaerosol
viability during the sampling processes was
highly influenced by bioaerosol
characteristics (hardy or fragile), as well as
by the fluorescent dyes with different
physiological mechanisms. For better
viability of the sampled bioaerosol, the
impinger was superior to the filter.
Moreover, it was found that sampling stress
from filtration had more influence on the
bioaerosol metabolism mechanism than cell
membrane integrity. Furthermore, the
differences between cell membrane integrity
and the metabolism by sampling stress were
found related to the bioaerosol species.
Subjects
bioaerosol
sampling efficiency
flowcytometry
Publisher
臺北市：國立臺灣大學公共衛生學院環境衛生研究所
Type
report
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