Study on Bio-Mechanical Properties of Mouse Colon Carcinoma(CT26) Utilizing Magnetic Tweezers
Date Issued
2010
Date
2010
Author(s)
Shiao, Chih-Yuan
Abstract
Bio-mechanic is a subject of interest for understanding the living cell properties. Mechanical stresses on the cells affect the morphology and cytoskeleton structure of cells, and further contribute for cell migration, growth, differentiation and apoptosis. The goal of this thesis is to explore the mechanical behavior of cells utilizing a home-constructed magnetic tweezers, which with advantages of exerting a non-invasive force on living cells.
The experimental approach is that, at first, the living cell of mouse colon carcinoma, CT26, were incubated in media with superparamagnetic nano-particles of Fe3O4. Waiting for overnight, then, the nano-particles were uptake by cells. Further, the magnetic tweezers were utilized to exert magnetic force to the cell, and the motions were recorded by a CCD camera. As observed, the cell creeped and relaxed in subject to the repeated forces. The mechanical properties of cell varied systematically with magnitude of external applied forces. Derive from motion pictures, we were able to obtain the displacement of particles in function of time. Further, two models, viscoelastic solid model and power law model, have been applied to analyze the displacement. The results have depicted that cell creep function over three time decades in different magnitude of forces conformed to a weak power law. It is noticed that cells have shown a stiffening behavior when subjected to cyclic forces. The significance of the fitting parameters obtained from analyzed experimental with theoretic models will be discussed in this thesis. The results have demonstrated that magnetic tweezers is a potential techniques for studying bio-mechanics of living cells.
Subjects
Magnetic tweezers
creep function
viscoelastic
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