Preparation and Characterization of Nano/Submicron Particles from Ganoderma Fruiting Bodies
Date Issued
2012
Date
2012
Author(s)
Lin, Ying-Sheng
Abstract
The preparation and stability of nano/submicrometer-sized Ganoderma tsugae (G. tsugae) was explored. The fruit body of G. tsugae was treated by homogenization and media-milling. Three sizes (0.8, 0.3, and 0.2 mm) of media were employed for three consecutive milling (I, II and III). The supernatant was considered as final product after centrifuge (10,000 g, 10 min). The removal of large particles resulted in better stability. Particle size was determined by using a laser-light particle size analyzer and further examined by using transmission electron microscopy. After being stored at 25oC for 21 days, the volume-average diameter of supernatant III remained below 0.2 μm with good stability. Freeze-drying resulted in an increase in particle size due to aggregation. Twenty percents of the particles in the freeze-dried supernatant III were smaller than 100 nm, with an average diameter greater than 1 μm. Nevertheless, autoclaving did not result in severe instability of supernatant III. After being stored at 25oC for 12 months, about 40% of the particles were smaller than 100 nm, with an average diameter of 373 nm. In addition, the contents of bioactive compounds such as β-(1,3)-D-glucans, crude triterpenoids, total dietary fiber and chitin in media-milled products were much greater than those present in hot-water extract. The media-milled products from G. tsugae showed moderate antioxidant activities at high concentration, and total phenols were the major naturally occurring antioxidant component. The anti-proliferation effect was observed in vitro growth of HepG2 and A375 cell lines after incubation with Supernatant III.
Subjects
nano/submicron
media milling
stability
bioactivity component
antioxidant properties
anticancer activity
SDGs
Type
thesis
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