Drosophila decapping protein 2 modulates the formation of cortical F-actin for germ plasm assembly
Journal
Developmental Biology
Journal Volume
461
Journal Issue
1
Pages
96 - 106
Date Issued
2020
Author(s)
Lee, Yi-Mei
Chiang, Po-Hsun
Cheng, Jen-Ho
Shen, Wei-Hong
Chen, Chao-Han
Wu, Mei-Ling
Tian, Yi-Lu
Ni, Chao-Heng
Wang, Ting-Fang
Lin, Ming-Der
Abstract
In Drosophila, the deposition of the germ plasm at the posterior pole of the oocyte is essential for the abdomen and germ cell formation during embryogenesis. To assemble the germ plasm, oskar (osk) mRNA, produced by nurse cells, should be localized and anchored on the posterior cortex of the oocyte. Processing bodies (P-bodies) are cytoplasmic RNA granules responsible for the 5′–3′ mRNA degradation. Evidence suggests that the components of P-bodies, such as Drosophila decapping protein 1 and Ge-1, are involved in the posterior localization of osk. However, whether the decapping core enzyme, Drosophila decapping protein 2 (dDcp2), is also involved remains unclear. Herein, we generated a dDcp2 null allele and showed that dDcp2 was required for the posterior localization of germ plasm components including osk. dDcp2 was distributed on the oocyte cortex and was localized posterior to the osk. In the posterior pole of dDcp2 mutant oocytes, osk was mislocalized and colocalized with F-actin detached from the cortex; moreover, considerably fewer F-actin projections were observed. Using the F-actin cosedimentation assay, we proved that dDcp2 interacted with F-actin through its middle region. In conclusion, our findings explored a novel function of dDcp2 in assisting osk localization by modulating the formation of F-actin projections on the posterior cortex.
SDGs
Type
journal article