Influence of media milling on water-soluble polysaccharides from Lycium barbarum
Date Issued
2011
Date
2011
Author(s)
Tu, Wen-Chin
Abstract
Lycium barbarum L. has multiple biological and pharmacological activities and has been used as a Chinese medicine and food. Media milling has been successfully employed to prepare nano/submicron Lycium suspension (L. suspension) in our lab, and has no mutagenic effect to the Samonella typhiumurium TA98 and TA100. The objective of this study is to identify free sugar, oligosaccharides and polysaccharides components of media milling Lycium barbarum and to evaluate the effects of media milling on Lycium barbarum polysaccharides (LBP). Media milling for 90min, the number average diameter of L. suspension particle was reduced from 3.54 μm to 0.101 μm, and percentage of particle smaller than 0.1 μm was 62.32%. SEM observation also proved the existence of nano/submicron L. suspension particles. The carbohydrate content of the blended LBP and milled LBP were 59.98 mg/g of dry Lycium and 86.47 mg/g of dry Lycium; and uronic acid content was 30.43 mg/g of dry Lycium and 45.83 mg/g of dry Lycium. For both the blended and milled L. suspension, the monosaccharide composition was D-Glucose and D-Fructose; and the oligosaccharides were consisted of D-Fucose and a few D-Rhamnose, D-Arabinose and D-Galactose. The molecular weight of the blended and milled LBP had a widely distributed rang of 6 - 2000 kDa. Polysaccharides extracted from the blended and milled L. suspension consist of D-Galactose, D-Glucose, D-Arabinose, D-Xylose and D-Mannose in a molar ratio of 3.03 : 1.03 : 1 : 0.25 : 0.06, and 2.98 : 1.21 : 1 : 0.24 : 0.04, respectively. Blended and milled water-soluble polysaccharide can be separated into neutral polysaccharide of L. barbarum (LBPN) and acidic polysaccharide of L. barbarum (LBPA1 and LBPA2) fractions by DEAE. For the total carbohydrate content, the LBPN, LBPA1 and LBPA2 was 14.81 : 51.44 : 33.75 for blended LBP; and 14.16 : 55.71 : 29.68 for milled LBP, before deproteination. After deproteination, LBPN, LBPA1 and LBPA2 was 46.18 : 29.57 : 24.24 for blended; and 39.14 : 30.40 : 30.46 for milleded LBP. Media milling can increase free sugar, oligosaccharide and LPB content, but does affect the composition of free sugar and oligosaccharide. There is no significant difference in sugar composition, molecular weight and content of acidic polysaccharides of LBP. The result showed that water-soluble polysaccharides from L. barbarum can be released by media milling but the process dose not significantly affect their property and sugar composition.
Subjects
nanotechnology
media milling
monosaccharide
oligosaccharide
water-soluble polysaccharide
Lycium barbarum L.
Type
thesis
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