行政院國家科學委員會專題研究計畫成果報告:鉤端螺旋體快速檢測試劑之開發
Date Issued
2004
Date
2004
Author(s)
潘銘正
DOI
922622B002006CC3
Abstract
Leptospirosis is a widespread zoonotic disease that affects all mammals in different parts of
the world. Clinical recognition of leptospirosis is challenging, and the definitive serologic
diagnosis assay, the microscopic agglutination test (MAT) is the reference test for diagnosis,
however, it is time-consuming and requires the maintenances of several leptospiral strains to be
used as antigens, live organisms creating a risk of laboratory-acquired infections. We proposed a
rapid dipstick test reagent for the detection of Leptospira- specific antibodies in human serum
samples. The efficacy of the test was compared with MAT, which is redarded as gold standard. A
total of 192 human serum samples were analyzed by both dipstick and MAT, the sensitivity for
detection of leptospiral antibodies was 14.9%; specificity was81%. The agreement (κ) with
MAT was as low as 0.04. The results indicated that the recombinant LipL32 protein expressed
in Escherichia coli BL21 is not suitable for detection of IgM antibodies
Subjects
leptospirosis
dipstick test
recombinant LipL32
SDGs
Publisher
臺北市：國立臺灣大學獸醫學系暨研究所
Type
report
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