行政院國家科學委員會三年期專題研究計畫之第三年成果報告:SALMONELLA CHOLERAESUIS 之鐵調節基因與毒力表現(3/3)
Date Issued
2002
Date
2002
Author(s)
張照夫
DOI
902313B002298
Abstract
Salmonella choleraesuis is a common swine bacterial pathogen. The infected pigs show septicemia, enterocolitis, and pneumonia. S. choleraesuis is also an important zoonotic pathogen. The molecular pathogenesis of S. choleraesuis is not clear. In S. typhimuriuin, many virulence genes, clustered together on chromosome, are Salmonella pathogenicity island (SPI). SPI-1 and SPI-2 encode structurally similar but functionally distinct type III secretion systems. The SPI-1 plays a role in invasion of epithelial cells. The SPI-2 is required for bacterial proliferation in macrophage and systemic infection. This study proved that S. choleraesuis had both of SPI- I and SPI-2 by Southern hybridization and polyrnerase chain reaction. The PCR products of hilA and spiCAB genes of S. choleraesuis were cloned and sequenced. These genes had high homology with S. typhimurium. The hilA mutant and spiC mutant were constructed by allelic exchange. The LD50 of hilA mutant was similar to that of wild S. choleraesuis. The hilA mutant was less invasive for HeLa cell, however, spiC mutant had the same invasion ability with wild type. These data paved the way to develop live S. choleraesuis vaccine.
Subjects
Salmonella choleraesuis
Salmonella pathogenicity island
allelic exchange
pat hogenesis
SDGs
Publisher
臺北市：國立臺灣大學獸醫學系暨研究所
Type
report
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902313B002298.pdf
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