Proteomic analysis of Rhodococcus pyridinivorans (PDB9) during biodegradation mechanism of Bisphenol A (BPA)
Date Issued
2012
Date
2012
Author(s)
Wong, Kam-Fei
Abstract
Bisphenol A (BPA) has been reported has an estrogenic activity and toxicity to aquatic organisms. This study was conducted to show the isolation and property of Rhodococcus pyridinivorans(PDB9) having BPA biodegradability. The bacterial strain was able to grow in a basal mineral salt medium containing BPA (50 mg/L) as the sole carbon source, and to degrade 90% BPA within 48 h. The effects of BPA on the biology of Rhodococcus pyridinivorans(PDB9) were elucidated using 2D proteomic. The cytoplasmic proteins isolated from these BPA –treated and -untreated cells after 24, 48hour were carried out for proteomic analysis. Four intermediated compounds were detected using Liquid Chromatography/Mass Spectrometry when BPA was degraded by Rhodococcus pyridinivorans via a novel pathway. There were five differential protein, respectively, as compared to their corresponding control (without BPA addition), at the indicated incubation times when 40% in 24h and 90% 48h of BPA had been removed. The five protein ID were identified by LTQ-FT-MS/MS. Proteins identified included NDMA-dependent methanol dehydrogenase, Elongation factor Tu, Cysteine synthase, Electron transfer flavoprotein beta/alpha subunit, ATP-dependent Clp protease proteolytic subunit. We had isolated a potential bacteria Rhodococcus pyridinivorans which could almost completely degrade 50 mg/L BPA within 48 hours. Moreover, using LC-Mass, we discovered a novel pathway to degrade BPA by microbe. Characterizing the bacterial response to BPA at the biochemical level identifies proteins that can be used by biocatalysts for biodegradation deserving further investigations.
Subjects
2D proteomic
Biodegradation
LTQ-FT-MS/MS
Bisphenol A
HPLC.
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-101-R98b46034-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):ceb4c0119590ff1585ef3789634709d3