Effects of Hyaluronan and Hydroxyapatite-containing Collagen Composite Matrices on the Proliferation and Osteogenic Differentiation of Adipose-derived Stromal Cells
Date Issued
2007
Date
2007
Author(s)
Chen, Po-Yang
DOI
zh-TW
Abstract
Suitable cell sources with good expansion technique, as well as excellent scaffolds are important for tissue engineering applicability. In this study, we aimed at the application of bone tissue engineering that the feasibility of in vitro expansion of adipose-derived stromal cells was investigated, and a tissue-engineered scaffold with osteoinductive characteristics was also developed.
First of all, hyaluronan was applied in this study to investigate its effect on the behaviors of mADSCs. It was shown in our results that as a medium supplement, hyaluronan could promote the proliferation and differentiation of mADSCs, and delay the build-up of senescence programs as well. On the other hand, upon culturing on hyaluronan-coated surface, mADSCs seemed to be quiescent without losing its proliferative and differentiation capacity as shown from the results that when a transfer of culture from quiescent state to regular culture environment was performed, the proliferative and differentiation potentials were restored, indicating that hyaluronan-coated surface could prevent the senescence and maintain the proliferation and differentiation of mADSCs.
In addition, being the predominant component of the inorganic substances in bone matrix, hydroxyapatite was proved to promote the proliferation and differentiation of bone cells. Collagen, the important structural protein of extracellular matrix, has been broadly utilized to prepare cell culture scaffolds due to its excellent biocompatibility. The porous matrix composed of both hydroxyapatite and collagen can therefore be used in bone cell culture in vitro as well as bone tissue regeneration in vivo.
Thus, we were instigated to fabricate the hydroxyapatite nanoparticles by wet-milling and they were then combined with collagen and hyaluronan which was capable of regulating various cell physiological behaviors to form a porous scaffold via phase separation technique. Our results demonstrated that the porous hydroxyapatite/hyaluronan/collagen composite matrix was suitable for cell proliferation in vitro, and it could also possess the osteoinductive effect that it was shown to promote the differentiation of murine adipose-derived stromal cells (mADSCs) towards osteogenic lineage without the administration of induction medium.
Subjects
透明質酸
羥基磷灰石
脂肪基質細胞
體外擴增
骨分化
hyaluronan
hydroxyapatite
adipose-derived stromal cells
in vitro expansion
osteogenic differentiation
Type
thesis
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