Crosslinking Of The Electrospun Human Hair Keratin-Gelatin Composite Film For The Promotion Of Peripheral Nerve Regeneration
Date Issued
2011
Date
2011
Author(s)
Chen, Yen-Yu
Abstract
The management of peripheral nerve injuries(PNI) with segmental defects is a challenge to surgeon. Clinical experience has shown that damaged nerve can be surgically repaired using a tubular conduit. Keratin from hair has been proposed as an appropriate material that supports cell adherence and improves cell growth. In this study, we focused on the development of artificial nerve guides that could offer better biocompatibility and mechanical ability during surgery and prolonged period of nerve regeneration; however, keratin which is not able to form fiber alone by electrospun. We used gelatin to mix with keratin for better viscosity. With the use of the electrical spinning technique to produce keratin-gelatin mixture fibers in random and parallel direction. Formic acid used as the dissolving solvent. Glutaraldehyde(GTA) as cross-linker to conjugate gelatin with keratin after electrospun. The bioactivity of both gelatin and keratin support a biocompatible environment for nerve. With the Microtensile test we wish to found the increase percentage of keratin may make better improvement of intensity which simply gelatin is poor and easy to hydrolyze. With the FTIR analysis to found what cross-linker make up chemical structure change .The cell culture and biodegradable testing biological activity of keratin-gelatin nanofibers films react to nerve cell. The morphology of the fibers was study by Scanning electron microscopy (SEM) and laser confocal microscopy observations the cells favorably and grow. Finally ,running RT-PCR to observe Schwann cell’s gene expression.
Numerous biodegradable materials have been used to make nerve conduit to repair injured nerves. We are now creating a novel biodegradable keratin-gelatin cross-linked conduit which we hope it can facilitate an outcome comparable to autograft in a nerve injury model.
Subjects
Keratin
Electrospun
Peripheral Nerve Regeneration
Type
thesis
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