Measurements of Nanobead’s Brownian Motion for the Detection of Cardiovascular Diseases Risk Factors
Date Issued
2009
Date
2009
Author(s)
Liu, Yi-Hsing
Abstract
Both the viscosity and the C-reactive protein (CRP) concentration of the serum are the symptoms of risk factors for cardiovascular diseases (CVD). In this study, a bio-sensing technique was employed to detect the viscosity and the CRP concentration of the serum by measuring nanobeads’ Brownian motion. Micro-Particle-Tracking-Velocimetry (μ-PTV) technique and Total Internal Reflection Fluorescence (TIRF) technique were used to obtain the characteristics of nanobeads’ Brownian diffusion. The results showed that as the viscosity increases 1cP, the nanobeads’ Brownian velocity was found to decrease about 10%. During the binding process of CRP onto nanobeads, the mean value of the beads’ diameters increases so that the Brownian velocity decreases with an increase of time. Moreover, higher CRP concentration leads to a lower Brownian velocity of the nanobeads in the equilibrium state. In the future, this simple sensing technique can be used to detect other bio-molecules and applied to the clinical assay of cardiovascular diseases risk factors.
Subjects
Brownian motion
C-Reactive Protein (CRP)
Cardiovascular Diseases (CVD)
Micro-Particle-Tracking-Velocimetry (PTV)
Total Internal Reflection Fluorescence (TIRF)
SDGs
Type
thesis
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