Analysis of gnrh3 structure, function and development in the zebrafish brain, Danio rerio.
Date Issued
2005
Date
2005
Author(s)
Kuo, Ming-Wei
DOI
zh-TW
Abstract
Expression of gnrh3 neurons started in the olfactory pit at 24-26 hours post-fertilization (hpf) and migrated into forebrain region during early larvae stage. We generated transgenic zebrafish expressing green fluorescent protein (GFP) and LacZ driven by the gnrh3 promoter. In gnrh3-transgenic fish, the expression of GFP and LacZ were similar to gnrh in the olfactory placode, olfactory bulb and telencephalon. To understand the function of GnRH at gnrh3 neuron development, we have done the morpholino knockdown and over-expression analysis. Reduced GFP and gnrh3 cell numbers, and delayed migration were caused by gnrh3 morpholino knockdown. The gnrh2 can rescue the morphants partially. Over-expression of gnrh3 can induce the earlier development of gnrh3 neurons. The gnrh3 expression was not in cyc, oep and smu mutant. PKI misexpression can rescue the gnrh3 defect in oep mutant. PKA and PKI misexpression caused the GFP cell numbers decrease and GFP ectopic expression, respectively. The normal gnrh3 expression and PKA pathway were involved in the development of gnrh3-expressing neurons during embryos. Transgenic zebrafish with specific gnrh3-GFP-LacZ expression in the brain offers a genetic tool to study the origin and migration of gnrh3 neuron lineage.
Subjects
性釋素
斑馬魚
基因轉殖
嗅板
gnrh
zebrafish
PKA
olfactory placode
Type
other