An animal study of applying amniotic membrane matrix for root coverage surgery
Date Issued
2010
Date
2010
Author(s)
Peng, Kuan-Yen
Abstract
Gingival recession is the most common mucogingival deformity in adult orthodontic patients. Treatment of gingival recession has been an important therapeutic issue due to increasing cosmetic demand. The exposed roots could be covered by various surgical procedures including connective tissue graft, free gingival grafts, coronally positioned flaps, and the use of barrier membranes. The amniotic membrane, the innermost layer of the fetal membrane, contains growth factors and large amount of collagen matrix. Therefore, amniotic membrane has been successfully used as a scaffold for cell growth and as a physiological wound dressing. The aim of this study was to assess the effect of applying de-epithelialized amniotic membrane in root coverage surgery to promote tissue regeneration. In a split-mouth design, the maxillary and mandibular third incisors, canines, second premolars, third premolars, and maxillary first premolars of 3 adult mongrel dogs were used. The buccal osseous dehiscence and gingival recession were surgically created 1 month prior to root coverage surgery, and refined immediately before the surgery. The exposed root surfaces were covered with de-epithelialized human amniotic membrane, and followed by the coronally positioned flap (experimental group). On the contralateral side, the coronally positioned flap was conducted without coverage of amniotic membrane (control group). The clinical parameters assessed during the experimental period included the probing depth and the amount of gingival recession (n =4~5). The observation time points were the 3 days, 2 weeks, 1 months, 2 months, and 6 months after root coverage. After the animals were sacrificed, the specimens of dento-periodontal complex were processed for paraffin sections and stained with haematoxylin-eosin. Histomorphometric evaluations were conducted by analyzing the parameters including the amount of new cementum formation, new bone formation, connective tissue regeneration, root coverage, epithelium thickness and height. Our results revealed that the amniotic membrane may inhibit tissue inflammation and promote rapid epithelialization at the initial healing of the gingival wound. The histomorphometric analysis showed that the periodontal tissue regeneration and root coverage in the experimental group were better than those in the control group. However, the group differences were not statistically significant. Further studies of higher sample size are needed to clearly assess the beneficial effect of applying amniotic membrane in the periodontal surgical procedures for root coverage.
Subjects
root coverage
amniotic membrane
periodontal tissue regeneration
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-99-R96422025-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):3baba289a018ff18b669124c1d708e10