Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning
Journal
STAR Protocols
Journal Volume
3
Journal Issue
2
Date Issued
2022
Author(s)
Borah B.J
Abstract
A resonant-scanning multiphoton optical microscope (MPM) with a millimeter-scale field-of-view (FOV) often encounters a poor Nyquist figure-of-merit (NFOM), leading to an aliasing effect owing to limited effective voxel-sampling rate. In this protocol, we provide a design guideline to enable high-NFOM MPM imaging while simultaneously securing a large FOV/digital-resolution ratio and a fast resonant raster-scanning speed. We further provide a free version of our custom acquisition software to assist with a smooth and easy construction process. For complete details on the use and execution of this protocol, please refer to Borah et al. (2021). © 2022 The Authors
Subjects
Biotechnology and bioengineering; Cell Biology; Microscopy
SDGs
Other Subjects
multiphoton microscopy; procedures; scintiscanning; software; Microscopy, Fluorescence, Multiphoton; Radionuclide Imaging; Software
Type
journal article