Synthesis and photodynamic efficacy of PAMAM-photosensitiser conjugates in vitro
Journal
2006 NSTI Nanotechnology Conference and Trade Show
Journal Volume
2
Pages
384-387
Date Issued
2006
Author(s)
Abstract
Starburst polyamidoamine (PAMAM) dendrimer is a new class of highly branched spherical polymer. Covalent attachment of porphyrin to PAMAM dendrimer may alter the pharmacokinetics, cell targeting, and photophysical properties of porphyrin. We investigated the uptake, localization, and phototoxicity of PAMAM-porphyrin conjugate with different charges and hydrophobicity in Hela cell lines. The cellular uptake of PAMAM-porphyrin conjugate for HeLa cells was much higher than that of native porphyrin. PAMAM-porphyrin conjugate was accumulated in the endosomal/lysosomal compartments in HeLa cells, and presented more effective photocytotoxity after irradiation. The Hydrophobic porphyrin conjugated to PAMAM showed less aggregation, more cellular uptake, and enhanced lysosomal targeting that could be applied on photosensitizer delivery system in clinical cancer therapy.
Subjects
Photodynamic therapy; Polyamidoamine dendrimer; Polymer-durg conjugate
SDGs
Other Subjects
Amines; Cells; Hydrophobicity; Pharmacokinetics; Synthesis (chemical); Toxicity; Cellular uptake; Polyamidoamine dendrimer; Polymer-durg conjugate; Porphyrin; Dendrimers
Type
conference paper