The use of polyethylenimine-DNA to topically deliver hTERT to promote hair growth
Resource
Gene Therapy , 19, 86-93
Journal
Gene Therapy
Journal Issue
19
Pages
86-93
Date Issued
2011
Date
2011
Author(s)
Abstract
The present study investigates the efficacy of polyethylenimine (PEI)-DNA complex that expressed human telomerase reverse transcriptase (hTERT) to transfect hair follicle stem cells and produce sufficient hTERT to stimulate hair growth. Transfection with pLC-hTERT-DNA-PEI complex (DP group) in vitro induced expression of proliferating cell nuclear antigen in 35.8% of the purified stem cell population, suggesting enhanced cell proliferation. In vivo transfection efficiency of rat dorsal skin was determined by staining for Β-gal activity. Cells positive for Β-gal were located in the bulge region and dermal sheath of hair follicles. The follicles in the hTERT-transfected region entered anagenon day 15 after transfection, whereas non-transfected (Neg) controls remained in telogen. The similar effect was observed in 50-day-old rat dorsal skin. D+P group displayed a specific expression of hTERT and sufficient to initiate a transition to the anagen phase and promote new hair synthesis 18 days after the transfection. hTERT promoted follicle neogenesis following wounding. In all, 60 days after wounding, tissues of the D+P group showed more newly regenerating hair follicles (83+52 regenerated follicles per rat) in contrast to control group tissues (15+15 regenerated follicles per rat). These studies provide a potential approach for gene therapy of skin disease. ? 2012 Macmillan Publishers Limited All rights reserved.
SDGs
Other Subjects
beta1 integrin; CD71 antigen; cycline; DNA; polyethyleneimine; telomerase reverse transcriptase; animal cell; animal experiment; animal tissue; antigen expression; article; cell proliferation; controlled study; cytotoxicity; genetic transfection; hair follicle; hair growth; hypothesis; in vitro study; keratinocyte; nonhuman; priority journal; rat; synthesis; Animals; beta-Galactosidase; Cell Proliferation; Cell Survival; DNA; Flow Cytometry; Gene Therapy; Gene Transfer Techniques; Hair Follicle; Humans; Keratinocytes; Mice; Plasmids; Polyethyleneimine; Proliferating Cell Nuclear Antigen; Rats; Rats, Wistar; Regeneration; Stem Cells; Swiss 3T3 Cells; Telomerase; Time Factors; Transfection; Wounds and Injuries; Rattus
Type
journal article
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