以雷射捕捉顯微分離法研究肝星狀細胞基因之表現
Other Title
Study of gene expression of hepatic stellate cell
using laser capture microdissection
using laser capture microdissection
Date Issued
2000
Date
2000
Author(s)
李嘉哲
DOI
892315B002025
Abstract
Liver
cirrhosis is characterized by pathol
ogical deposit of collagen and other
excellular
matrix proteins resulting in disrupt
ion of normal liver architecture and
a compromise in liver function.
These matrix proteins are synthesize
d by activated hepatic stellate cell
s (HSC).The accumulation of
matrix proteins may reflect increased
matrix synthesis and/or decreased
matrix degradation.Matrix
metalloproteinases (MMP)
are able to degrade all
of the components of extracellular
matrix and are the major enzymes in
matix turnvoer.Specific inhibition of
MMPs occurs by interaction
with the tissue inhibitors of matrix
metalloproteinases (TIMP).
The activated hepatic stellate cells
(HSC)
is known to secrete procollagen,thr
ee MMPs and two TIMPs:interstitial
collagenase (MMP-1),gelatinase A
(MMP-2)and stromelysin (MMP-3),
TIMP-1 and TIMP-2.In this project
we tried to isolate HSC using laser
capture microdissection (LCM)to study the expression of
MMPs and
TIMPs.We tried both frozen liver ti
ssue and formalin-fixed tissue.
PixCell Laser Capture Microdissection
System captured about 5 cells in
each laser excitation.RTPCR with
actin primer only succeeded
in frozen tissue.The detection limit
is about 100 cells.We used anti-smooth
muscle actin and anti-desmin
antibody to indentify HSC.HSC can
only be stained in formalin-
fixed tissue.The filamentous-
shaped HSC made the capture of pure cell
impossible.We concluded that
at present time using LCM to isolate
HSC for study expression of MMP and TIMP
is not feasible.
Subjects
Liver cirrhosis
Stellate cell
Matrix
metaloproteinase
metaloproteinase
Publisher
臺北市：國立臺灣大學醫學院內科
Type
report
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