Recombinant Coat Protein Suppress Whitefly Transmission of Tomato yellow leaf curl Thailand virus
Date Issued
2015
Date
2015
Author(s)
Tseng, Chien-Hao
Abstract
Whiteflies not only damage plants through feeding but also serve as vectors transmitting plant viruses. Whitefly-transmitted begomoviruses threaten a wide range of crops worldwide. Tomato yellow leaf curl Thailand virus (TYLCTHV) and Tomato leaf curl Taiwan virus (ToLCTWV) are two most prevalent viruses in tomato fields in Taiwan. Coat protein (CP), the only structural protein of begomoviruses, is hypothesized to interact with a putative receptor in the midgut of Bemisia tabaci. In vivo binding assay demonstrated recombinant TYLCTHV CP specifically bound to the midgut cells of B. tabaci. The objective of this study was to determine if preacquisition of TYLCTHV CP competes the putative receptor in the midgut of B. tabaci hence suppresses the acquisition and vector transmission of TYLCTHV. Preacquisition of recombinant TYLCTHV CP decreased the accumulation level of TYLCTHV in the midgut cells of B. tabaci but did not reduce the acquisition of TYLCTHV in B. tabaci. Further, preacquisition of recombinant TYLCTHV CP suppressed the whitefly transmission of TYLCTHV. Similar experiments were conducted to investigate if preacquisition of TYLCTHV CP (homologous to ToLCTWV CP) would reduce virus accumulation and acquisition in B. tabaci, and the whitefly transmission of ToLCTWV. The results demonstrated that preacquisition of recombinant TYLCTHV CP did not influence the accumulation and acquisition of ToLCTWV in B. tabaci and did neither reduce the whitefly transmission of ToLCTWV. In conclusion, TYLCTHV CP functioned as an attachment protein and preacquisition of TYLCTHV CP reduced the whitefly transmission of TYLCTHV. The failure that using TYLCTHV CP to inhibit the whitefly transmission of ToLCTWV suggested that the interaction between TYLCTHV CP and the putative receptor in the midgut of B. tabaci is specific. Understanding the CP-midgut interaction would unveil the mechanism of whitefly transmission of begomoviruses and warrant further study to develop strategies for disease control.
Subjects
Bemisia tabaci
recombinant viral protein
vector transmission
immunofluorescence assay
Type
thesis
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