Expression analysis of β-1,3-glucanase gene of Lilium cv. Star Gazer
Date Issued
2006
Date
2006
Author(s)
Chang, Whei-Shin
DOI
zh-TW
Abstract
In the study of the interaction between Botrytis elliptica and Lilium cv. Star Gazer, analysis of recovery of inoculation fluids and a subsequent cDNA cloning identified the LPGlu1 protein (35.34 kDa, pI 4.14), sharing high similarity to various plant acidic β-1,3-glucanases. Through PCR technique, specific primers to lpglu1 could be used to amplify relevant fragment from L. longiflorum (hybrid) and L. formosanum genome. The appropriate annealing temperature is between 54-57℃ and 55℃, and the annealing temperature of lpglu1 gene in Star Gazer is about 57-60℃. LPGlu1 without signal peptide could be expressed in Escherichia coli XL1-Blue strain by pQE 30 protein expression system. His-lpg displayed β-1,3-glucanase activity of 7.34 U/mg. The expression of lpglu1 in ‘Star Gazer’ leaves were investigated by RT-PCR and Southern hybridization. The results indicated that the accumulation of lpglu1 mRNA could be induced by B. elliptica, salicylic acid, methyl jasmonate, abscisic acid and 1-aminocyclopropane-1-carboxylic acid. In addition, wounding, cold and high salt could induce the expression of lpglu1 gene.
Subjects
百合灰黴病菌
系統性誘導抗病
葵百合
β-1,3-葡聚醣酶
病程相關蛋白質
Botrytis elliptica
B. cinerea
systemic acquired resistance
Lilium cv. Star Gazer
β-1,3-glucanase
pathogenesis-related proteins
Type
other
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