Establishment and Evaluation of Ca9-22 Cell Platform for Screening Food Factors with Anti-oxidative Potential
Date Issued
2014
Date
2014
Author(s)
Hsu, Yu-Ting
Abstract
Reactive oxygen species (ROS) act as signaling intermediates for many normal cellular processes, but elevated ROS has been linked to over 150 diseases, including atherosclerosis, diabetes, cancer, and neurodegenerative diseases. These free radicals generated from living body or environment are sources of oxidative stress. Severe oxidative stress leads to DNA damage or/and cell death. Nuclear factor-erythroid 2-related factor-2 (Nrf2) is a key transcription factor that plays a central role in cellular defense against oxidative and electrophilic damages by induction of anti-oxidative enzymes, including heme-oxygenase 1 (HO-1) and NAD(P)H: quinone oxidoreductase-1 (NQO1). The objective is to establish a cell platform to screen food factors with potential anti-oxidative function. Our strategy is to construct a plasmid that contains antioxidant responsive element (ARE) driven promoter and secretory form of luciferase reporter genes, and followed by transfecting the plasmid to oral cancer cell line Ca9-22. We selected stable lines with the reporter plasmid insertion in chromosome and evaluate the efficacy of the cell platform by Bracteanolide A, a natural compound that we have previously identified as a strong antioxidant, which could prevent cell from oxidative stress and strongly drive Nrf2 and downstream target genes. We have successfully constructed this cell platform and confirm its stability and sensitivity. In the future, it could be a good tool toward screening food factors with potential antioxidant acitivities.
Subjects
抗氧化
細胞平台
分泌型報導基因 (Secretory luciferase reporter gene)
抗氧化反應片段(Antioxidant responsive element, ARE)
NF-E2相關因子二 (Nuclear factor-erythroid 2-related factor-2)
Ca9-22
SDGs
Type
thesis
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