Using High-Throughput Single Nucleotide Polymorphism Genotyping to Reveal Litter Size-Related Molecular Markers in Landrace Sows
Date Issued
2010
Date
2010
Author(s)
Chang, Pin
Abstract
Reproduction performance of sows is one of the most important factors for pig industry, which is the mainstream of livestock production in Taiwan. Improvement of the litter size, a critical reproduction trait, by traditional genetic selection methods generates limited effect, due to the low heritability and sex-limited trait. Using single nucleotide polymorphism (SNP) molecular markers for a marker-assisted selection program through candidate gene approach may improve reproductive traits. The purpose of this study was to investigate the genetic polymorphisms in candidate genes and their association with reproductive performance in Landrace sows, the major breed used as dam in commercial pig production in Taiwan.
Candidate genes were selected from embryonic mRNA differentially expressed between morula and blastocysts. After cloning and sequencing the promoter region of the 19 candidate genes, we found 92 novel SNPs from DNA sequences of 30 unrelated Landrace pigs. There are 78 SNPs located on regulatory region, 11 SNPs on the 5’ UTR, 2 SNPs on the intron and 1 SNP located on the CDS. Furthermore, by using the high-throughput Illumina GoldenGate® genotyping assay, we found 10 novel SNP markers significantly associated with total number born (TNB) and number born alive (NBA), including genes of activating signal co-integrator 1 complex subunit 3 (SSC.13633-B, P < 0.05), LIM and SH3 protein 1 (PLAb11012-C, P < 0.05; PLAb11012-E, P < 0.01), ataxia-telangiectasia mutated（SSC.21876-A, P < 0.05; SSC.21876-L, P < 0.01）, plasma platelet-activating factor acetylhydrolase（SSC.19691-A, P < 0.05; SSC.19691-F, P < 0.01）, serum amyloid A（SAA-Q, P < 0.05 ; SAA-AA, P < 0.05）, and Visfatin (Visfatin-A, P < 0.05) in 460 Landrace sows from two commercial breeding herds. These litter size-related SNPs are good candidate molecular markers for improving the reproductive efficiency of pigs.
The present study developed an effective platform for determining the litter size-related SNPs, which might be associated with the regulatory region of differentially regulated genes from morula to blastocyst. Once validated further with large sample size and collected fully-formed records from different herds that would be applied to marker-associated selection to improve reproductive performance in Landrace sows, and increased the production value on the pig production industry.
Subjects
Landrace sows
Litter size
Single nucleotide polymorphism
Early embryo
High-throughput genotyping
Differentially expressed gene
LIM and SH3 protein 1 gene
activatin signal cointrgrator 1 complex subunit 3 gene
Ataxia-telangiectasia mutated gene
Plasma platelet-activating factor acetylhydrolase gene
serum amyloid A gene,
Visfatin gene
Type
thesis
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