水稻營養器官中澱粉代謝基因表現之研究(I)水稻葉鞘中澱粉代謝之調控 機制(1/3)
Date Issued
2005-07-31
Date
2005-07-31
Author(s)
王淑珍
DOI
932313B002070
Abstract
Starch accumulated in rice (Oryza sativa L.) leaf sheath before heading and degradation for
providing carbon source to panicles after heading is an important factor to affect the grain
yield. However, the mechanisms for controlling the starch synthesis and metabolism in leaf
sheath at the sink-source transition stage are still unclear. In this study, starch content
change during the heading period in the second leaf sheath (-2 leaf sheath) below flag leaf
was observed. In order to understand the regulatory mechanism of starch content change
during heading period, expressions of 18 genes involved in starch synthesis and degradation were identified during this period. Besides, expressions of several genes
related to nitrogen metabolism, sucrose biosynthesis and photosynthesis were also
determined in -2 leaf sheath. Among carbohydrate metabolism-related genes, the reverse
northern hybridization data showed that the mRNA level of ADP-glucose
pyrophosphorylase large subunit (AGPL) and small subunit (AGPS), granule-bound starch
synthase I (GBI), branching enzyme (BE) IV, soluble starch synthase(SSS) II-2, SSSIII,
SSSIV-1, SSSIV-2 were increased before heading and decreased after heading. The
transcript acumulations of BEIII, cytosolic and plastidic form of frutose-1,
6-bisphosphatase were increased at the time of heading. The results were suggested these
gene expressions could be related to the sink-source transition of -2 leaf sheath. On the
other hand, sucrose phosphate synthase (SPS) gene expressions were more active after
heading.
Publisher
臺北市：國立臺灣大學農藝學系暨研究所
Type
report
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