The Role of Calreticulin in the Pathogenesis of Neuroblastoma
Date Issued
2003-07-31
Date
2003-07-31
Author(s)
許文明
DOI
912314B002255
Abstract
Calreticulin(CRT) is a member of endoplasmic
reticulum protein. Our immunohistochemical results
show that the positive immunoreactive rate of CRT
increases as the histology becomes more
differentiated. Patients with positive expression of
CRT have a better overall survival rate. Our
preliminary results confirm that CRT plays an
important role in NB, and that CRT can be a
prognostic factor of NB. However, the real association
between CRT and differentiation of NB remains to be
clarified. To disclose the role CRT in the
pathogenesis of NB, this project comprises in vitro
studies by using NB cell lines. The protein levels of
CRT increased after NB cells were induced to
differentiate and remained high after the cells going
to apoptosis. The NB cells go to apoptosis after the
cells were transfected with CRT gene. Furthermore,
two-dimensional electrophoresis showed 39 proteins
increased and 40 decreased after the cells
differentiated, and 61 proteins increased and 22
decreased after the cells showing apoptosis. Our
results revealed that CRT plays roles both in the
differentiation and apoptosis of the NB cells. Further
study of the relationship between CRT and the
associated protein changes after the differentiation
and apotosis of the NB cells, may disclose the role of
CRT involved in the differentiation and apoptosis of
NB cells, and also its role in the pathogenesis of NB.
Subjects
Neuroblastoma
Pathogenesis
Cell differentiation
Apoptosis
Publisher
臺北市：國立臺灣大學醫學院外科
Type
report
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