Enantioseparation of Hydroxyflavanones in Cyclodextrin-Modified Capillary Zone Electrophoresis Using Sulfated Cyclodextrins as Chiral Selectors
Date Issued
2007
Date
2007
Author(s)
Fang, Wei-Ru
DOI
zh-TW
Abstract
In this study, enantioseparations of three hydroxyflavanone aglycones, including
2’-，3’- and 4’-hydroxyflavanone, in cyclodextrin (CD)-modified capillary zone electrohphoresis using randomly sulfate-substituted b-CD (MI-S-b-CD) or dual CD systems consisting of MI-S-b-CD and neutral CDs such as b-CD or g-CD as chiral selectors were investigated with a phosphate buffer at low pH. Sodium dodecyl sulfate (SDS) monomers were used as complexing agents for manipulating the selectivity of hydroxyflavanones. The results indicate that MI-S-b-CD is an excellent chiral selector
for enantioseparation of hydroxyflavanone aglycones, especially for 2’-hydroxyflavanone. The enantioselectivity of hydroxyflavanones can be greatly
enhanced using dual CD systems. Effective enantioseparations of these three
hydroxyflavanones can be simultaneously achieved within 20 minutes with 2.0 % (w/v) MI-S-b-CD or dual CD systems in combination with the addition of SDS monomers at a concentration less than 2 mM.
Subjects
毛細管電泳
對掌分離
環糊精
類黃酮
黃烷酮
Capillary Zone Electrophoresis
chiral seperation
Cyclodextrins
flavnoids
flavanone
Type
thesis
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