Study Of Fetal Mice Dental Stem Cells Cultured With Fibroblast Growth Factor -9
Date Issued
2009
Date
2009
Author(s)
Lin, Yi
Abstract
The human dentition is indispensable for nutrition, communication and physiology. Caries, pulpitis, apical periodontitis and trauma may lead to loss of tooth, causing the problem of pronunciation, mastication, and appearance difficulities. Abiological tooth substitute that could replace lost teeth would provide a vital alternative to currently avalible clinical treatment. or years research, people have obtained much more knowledge about this field. By animal model of embryonic tooth germ, we can understand how tooth germ cells separation of embryo layer, culture and finally recombination into tooth-like organ. In this experiment, we use some specific growth factors, some genetic inhibition and genetic expression., etc. Main conception is hoping through recombination and regulation of tooth germ cells in vitro. Our final subject is to regenerate a valuable bio-engineering tooth. ccording to the recent research, they inerted four significant genes into fibroblast with genetic transfection technology which forms “IPS”cells (induced pluripotent stem cells). In experiment group, they can find many properties similar to embroyo-stem cells. By inserting genes into fibroblast, we could find an alternative way in organic-regeneration-medication.his study focues on observation of tooth regeneration and the effect of specific growth factor treated on tooth germ cells. We obtained tooth germ stem cells form embryonic day 14th mouse low jaw molars through stereo-microscope. Then we minced and separated impact tooth germ into epithelium and mesenchyme stem cells by enzyme treatment. In addition, we find out the proper concentration of fibroblast growth factor-9 by MTT assay. We cultured separated tooth germ cells in TRANSWELL system and set up five different points and two groups, one is control group, and the other is experiment group, to observe the difference in RNA expression, at last we can discuss the effect on mesenchyme cells treated with Fgf-9. To observe the histology we use Hematoxylin and eosin (H&E) paraffin section of organ culture for ten days in Fgf-9 medium to compare with control group.ur results demonstrated that: (1) According to MTT assay, mesenchyme cells perform highly effective under circumstances with 25 ng/ml Fgf-9 in medium. (2)The expression of mesenchyme calcification-gene mRNA obviously increased from first day to sixteenth day co-cultured with 25 ng/ml Fgf-9 medium.(3)We can clearly distinguish the difference between control group and experiment group which proceed to secrete enamel-like matter.hese results suggest that: dental mesenchyme cells with odontogenic cells phenotype stimulated by fibroblast growth factor-9 could promote odontogenic gene expression. Whether or not fibroblast growth factor-9 can effectively promote and accelerate the regeneration of recombination-tooth needs more detailed study.eywords: fibroblast growth factor-9 , dental stem cells , mesenchyme cells , odontogenic , organ culture.
Subjects
fibroblast growth factor-9
dental stem cells
mesenchyme cells
odontogenic
organ culture
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