Nuclear condensation and cell cycle arrest induced by telomerase siRNA in neuroblastoma cells
Resource
J. Neuro-Oncol., 111(3), 265-272
Journal
Journal of Neuro-Oncology
Pages
265-272
Date Issued
2013
Date
2013
Author(s)
Tsai, Ming-Dar
Chen, Pei-Rong
Tien, Lu-Tai
Cai, Yu-Jyun
Lee, Yih-Jing
Abstract
Neuroblastoma is a type of malignant extracranial tumor that occurs in children. Advanced neuroblastoma, and tumors with MYCN amplification in particular, have poor prognoses. Therefore, it is important to find an effective cure for this disease. Small interfering RNA (siRNA) disrupts gene function by specifically binding to target mRNA. In this study, we used siRNA against telomerase to treat neuroblastoma, to evaluate any anti-proliferative effect on these cells. We evaluated cell viability by WST-1 assay on neuroblastoma cells treated with or without telomerase siRNA. Nuclear condensation, an indicator for apoptotic cells, was determined by DAPI labeling following siRNA treatment. The effectiveness of telomerase siRNA on altering the neuroblastoma cell cycle was detected by flow cytometry. Our results indicated that telomerase siRNA reduces the viability of neuroblastoma cells and increases the percentage of cells in the cell cycle's sub-G1 phase. We found that telomerase siRNA increases the percentage of condensed DNA in neuroblastoma cells. In conclusion, using siRNA against telomerase could be further developed as a therapy for the treatment of neuroblastoma.
Subjects
siRNA
Telomerase
Neuroblastoma
Nuclear condensation
Cell cycle arrest
SDGs
Other Subjects
4',6 diamidino 2 phenylindole; DNA fragment; small interfering RNA; telomerase; small interfering RNA; telomerase; antiproliferative activity; apoptosis; article; cell assay; cell cycle arrest; cell cycle G1 phase; cell labeling; cell viability; chromosome condensation; flow cytometry; gene delivery system; human; human cell; neuroblastoma cell; cell cycle checkpoint; cell proliferation; dose response; drug effect; genetic transfection; genetics; metabolism; neuroblastoma; nucleolus; pathology; physiology; RNA interference; tumor cell line; Apoptosis; Cell Cycle Checkpoints; Cell Line, Tumor; Cell Nucleolus; Cell Proliferation; Dose-Response Relationship, Drug; Flow Cytometry; Humans; Neuroblastoma; RNA Interference; RNA, Small Interfering; Telomerase; Transfection