A set of GFP-based organelle marker lines combined with DsRed-based gateway vectors for subcellular localization study in rice (Oryza sativa L.)
Journal
Plant Molecular Biology
Journal Volume
90
Journal Volume
90
Journal Issue
1-2
Journal Issue
1-2
Pages
107-115
Start Page
107
End Page
115
ISSN
01674412
Date Issued
2016-01-01
Author(s)
Wu, Tsung-Meng
Lin, Ke-Chun
Liau, Wei-Shiang
Chao, Yun-Yang
Yang, Ling-Hung
Chen, Szu-Yun
Lu, Chung-An
Abstract
In the post-genomic era, many useful tools have been developed to accelerate the investigation of gene functions. Fluorescent proteins have been widely used as protein tags for studying the subcellular localization of proteins in plants. Several fluorescent organelle marker lines have been generated in dicot plants; however, useful and reliable fluorescent organelle marker lines are lacking in the monocot model rice. Here, we developed eight different GFP-based organelle markers in transgenic rice and created a set of DsRed-based gateway vectors for combining with the marker lines. Two mitochondrial-localized rice ascorbate peroxidase genes fused to DsRed and successfully co-localized with mitochondrial-targeted marker lines verified the practical use of this system. The co-localization of GFP-fusion marker lines and DsRed-fusion proteins provide a convenient platform for in vivo or in vitro analysis of subcellular localization of rice proteins.
Subjects
DsRed
GFP
Organelle marker lines
Oryza sativa
Protoplast
Subcellular localization
Publisher
Springer Netherlands
Type
journal article