Intranasal administration of tissue inhibitor of metalloproteinase 1 extracted from stem cells from human exfoliated deciduous teeth-conditioned medium improves neurological outcomes and alpha-synuclein elimination in Parkinson's disease mice
Journal
Journal of Dental Sciences
ISSN
1991-7902
Date Issued
2024-12
Author(s)
Abstract
Background/purpose: Parkinson's disease (PD) remains a challenging neurodegenerative disorder, requiring the exploration of innovative therapeutic strategies. This study assessed the neuroprotective effects of stem cells from human exfoliated deciduous teeth-conditioned medium (SHED-CM) in a rotenone-induced mouse model of PD. Materials and methods: The SHED-CM, fractionated and purified via Fast Protein Liquid Chromatography (FPLC) into “yPD01”, was administered intranasally to evaluate its impact on motor deficits, olfactory functions, and protein expressions in affected brain regions. Results: Upon intranasal delivery, yPD01 produced significant improvements to motor deficits and restoration of protein expressions associated with PD pathology, particularly in the olfactory bulb and substantial nigra. Intriguingly, the intranasal route exhibited efficacy akin to intravenous administration, highlighting its potential as a minimally invasive yet equally effective therapeutic approach. Further investigation indicated key components within yPD01, denoted as peaks P1 and P5, showcasing pivotal therapeutic effects. These components were linked to the interruption of alpha-synuclein aggregation and its clearance within specific brain cells affected by PD. Conclusion: Intranasally administered yPD01 shows neuroprotective potential for mitigating neurodegenerative symptoms, particularly in PD. The results provide valuable insights into potential mechanisms underlying the neuroprotective effects of SHED-CM and their implications for future therapeutic interventions in PD and related conditions. © 2024 Association for Dental Sciences of the Republic of China
Subjects
Alpha-synuclein aggregation
Parkinson's disease
Rotenone-induced animal model
SHED-CM
SDGs
Publisher
Association for Dental Sciences of the Republic of China
Type
journal article