Analysis of leaf volatile constituents of Pelargonium graveolens and P. capitatum cv. Attar of Rose -effect of leaf age and etiolation
Date Issued
2008
Date
2008
Author(s)
Liao, Jun-Ming
Abstract
The research utilizes solid-phase microextraction （SPME）and gas chromatography-mass spectrometry （GC-MS）to analysis P. graveolens and P. capitatum cv. Attar of Rose leaf volatile constituents. And research the volatile constituents of leaf in （1）effect of leaf age and （2）etiolation.Result of research establish terms of analysis at first, the best analysis terms is using 4~5 leaf discs and SPME to extract the volatile constituents for 4 minutes in 60℃. The result showed that the leaf age could influence leaf volatile constituents of P. graveolens and P. capitatum cv. Attar of Rose. The volatile constituents content of the leaf per unit area, including menthone, citronellol, geraniol, citronellyl formate, β-caryophyllene and 3,7-guaiadiene, decreased with the leaf age and the variation reached significant difference. The total volatile constituents content of the leaf would reach to maximum when the leaf were about three weeks old or four weeks old, and the leaf would become mature nearly. The total content of leaf volatile constituent started to reduce with leaf age when the leaf were five weeks old and full expanded to mature. As for the total leaf area per unit area, the content of trans-rose oxide would reach to maximum when the leaf were one week old and increased with the leaf age.In etiolation and non-etiolation treatment of P. graveolens and P. capitatum cv. Attar of Rose, the variation of SPAD reading and each volatile content had no significant difference. That showed there’s no obvious correlation between chlorophyll change and content of each constituent.In etiolation and non-etiolation treatment of P. graveolens, citronellol and geraniol; citronellyl formate and β-caryophyllene; citronellyl formate and 3,7-guaiadiene; β-caryophyllene and 3,7-guaiadiene, the variation of volatile constituent content to each other of these 4 groups didn’t reach significant difference. And it showed that etiolation could not effect the relation of these 4 groups. In etiolation treatment, trans-rose oxide and citronellol; trans-rose oxide and geraniol; menthone and citronellyl formate; menthone and β-caryophyllene; menthone and 3,7-guaiadiene, the variation between each other of these 5 groups reached significant difference. It showed that sunlight could effect these 5 groups of constituents.There were 3 groups constituents in etiolation and non-etiolation treatment of P. capitatum cv. Attar of Rose, including menthone and citronellyl formate; menthone and 3,7-guaiadiene; β-caryophyllene and 3,7-guaiadiene, and the variation between each other of these 3 groups reached significant difference. It showed that sunlight could not effect these 3 groups of constituents. In etiolation treatment the relation of menthone and β-caryophyllene became non-significant different, and in etiolation treatment, the relation of citronellyl formate and β-caryophyllene could effect by sunlight.In etiolation treatment and resumed to sunlight of P. capitatum cv. Attar of Rose leaf, the volatile constituents content of menthone, citronellol, citronellyl formate , β-caryophyllene, 3,7-guaiadiene would decrease by the days after treatment and the variation reached significant difference. It showed that etiolation resumed to sunlight could not restore the volatile constituent content. The correlation with SPAD reading and AI value of each constituent, only menthone reached significant difference when resumed to sunlight 2~5 days, the other constituents, such as citronellol, citronellyl formate, β-caryophyllene and 3,7-guaiadiene, did not reach significant difference.
Subjects
Pelargonium graveolens
Pelargonium capitatum cv. Attar of Rose
SPME
leaf age
etiolation
Type
thesis
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