Isolation Rate and Antimicrobial Resistance of Salmonella in Market Raw Oysters (Crassostrea gigas) in Northern Taiwan
Date Issued
2012
Date
2012
Author(s)
Chen, Pai-Hsung
Abstract
Salmonellosis frequently causes food-borne illness in human, and poses a serious public health concern. Many of the salmonellosis outbreaks are associated with the consumption of oysters. The research about oyster in Taiwan is absent. Hence we had collect oysters from northern Taiwan’s retail market and supermarket, totally 137 samples. Import ready-to-eat oyster, totally 114 samples and screened for the presence of Salmonella. Isolates were characterized by serotyping and antibiotic resistant testing. None of imported oysters showed positive results. The domestic samples were 80 Salmonella-positive where 91 Salmonella strain were isolated and 11 Salmonella serovars were identified. The top three are Saintpaul (26.4%), Newport (21.9%) and Infantis (13.2%). The most frequent resistances were to Tetracycline and Oxytetracycline (n=12, 14.3%). The antibiotic related genes: Integron, tetA, tetB, PSE-1 were detected in 16.5%, 26.4%, 6.6%, 22% respectively. According to statistics result, the contamination level in oyster rearing location, Dongshih’s isolation rate (68.8%) is significantly higher than Budai (20.0%) and Wanggang (9.1%) (P<0.05). The presence of Salmonella isolation rate is highly associated with soaked-in-water sold type (P<0.05). The Integron-positive strains are highly associated with the presence of the antimicrobial resistance. The tetA-positive strain are highly associated with the presence of the Tetracycline and Oxytetracycline resistance. and PSE-1-positive strain are highly associated with the presence of the Ampicill in resistance. In our result shows market raw oyster are still have potential risk in foodborne disease. The hazard points include rearing enviroment and transportation procedures. Consumers should be noticed to avoid cross-infection. Further researches of contamination source and transmission route are needed.
Subjects
Salmonellosis
Integron
gene cassette
SDGs
Type
thesis
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